In a six base pairs long restriction site of a restriction enzyme first 3 nucleotides starting from 5’ end are GAT. What will be the sequence of next three nucleotides?

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TAG

GAT

GTA

ATC

answer is D.

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Detailed Solution

In molecular biology, restriction enzymes typically recognize and cut DNA at specific sequences known as restriction sites. These sites are typically palindromic, meaning they read the same forward and backward on both DNA strands. When a restriction enzyme recognizes a specific sequence, it cuts the DNA at or near that sequence.

It is mentioned that the first 3 nucleotides in the restriction site are "GAT," which is read from the 5' end. To maintain palindromicity, the next 3 nucleotides on the complementary strand would be the reverse of "GAT," which is "ATC."

So, the sequence of the next three nucleotides on the complementary strand would be "ATC."

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