In bioprocess engineering 

A. sterile ambience is maintained in cultures

B. desirable microbes or eukaryotic cells are cultured in large quantities

C. hybridisation is carried out

Bioprocess engineering is a chemical engineering process in which desirable microbes are eukaryotic cells are grown in larger quantities in microbial contamination free ambience for large scale production of vaccines, enzymes, hormones, antibiotics etc. useful to mankind.