In Calvin cycle, RuBisCO incorporates ${\mathrm{CO}}_2$ into ribulose, 1, 5-bisphosphate which rapidly splits into

In Calvin cycle, carboxylation is the most crucial step where CO2 is utilised for the carboxylation of RuBP. In this CO2 reacts with ribulose 1,5 biphosphate to yield two molecules of 3-phosphoglycerate, a reaction catalyzed by the chloroplast enzyme ribulose biphosphate carboxylase-oxygenase, referred to as RuBisCO.