In Hershey and Chase experiment

a) Radioactive S³⁵ is detected in bacterial cell in sediment

b) Radioactive  P³² is detected in supernatant in viral capsid

c) Radioactive S³⁵ is detected in capsid in supernatant

d) Radioactive  P³² is detected in bacterial cell in sediment

In Hershey and Chase experiment Radioactive S³⁵ is detected in capsid in supernatant and radioactive  P³² is detected in bacterial cell in sediment.

In the Hershey-Chase experiment, radioactive sulfur-35 (³⁵S) was used to label the protein coat (capsid) of the T2 bacteriophage, and radioactive phosphorus-32 (³²P) was used to label the DNA of the bacteriophage.

After the bacteriophages were allowed to infect the bacterial cells and the protein coats were separated using a blender, it was found that the radioactive sulfur-35 (³⁵S) remained in the supernatant with the capsids, while the radioactive phosphorus-32 (³²P) was detected in the bacterial cell pellet. This result demonstrated that the genetic material responsible for directing the replication of new bacteriophage particles was indeed the DNA, as it entered the bacterial cells, while the protein coat remained outside.