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In recombinant DNA technology for the insertion of a foreign gene into a plasmid vector, the vector must be opened by treating it with

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Alkaline phosphatase only

RNA ligase

DNA polymerase

The same restriction enzyme used to cleave the foreign DNA

answer is D.

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Detailed Solution

When cut by the same restriction enzyme, the resultant DNA fragments of plasmid vector and foreign DNA have the same kind of ‘sticky-ends’ which can be joined together (end-to-end) using DNA ligases.

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