In the protocol of DNA fingerprinting, separation of DNA fragments by electrophoresis is preceded by

The steps in the DNA fingerprinting are in the sequence:

(i) isolation of DNA
(ii) digestion of DNA by restriction endonucleases
(iii) separation of DNA fragments by electrophoresis
(iv) transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon
(v) hybridisation using labelled VNTR probe
(vi) detection of hybridised DNA fragments by autoradiography.