In the technique of selection of transformed recombinant host cells by insertional inactivation method, identify the correct statement.

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$β$ -galactosidase gene is inactivated due to insertion of desired gene in the amino acid sequence of the enzyme $β$ -galactosidase

The enzyme $β$ -galactosidase is inactivated by the presence of desired alien gene in the medium

Colonies of normal host cells which do not contain an insert produce blue colour in the presence of suitable chromogenic substrate in the medium

Cells of blue coloured colonies turn colourless due to insertional inactivation of $β$ –galctosidase enzyme.

answer is D.

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Detailed Solution

Blue white colonies selection method helps to differentiate recombinants from non recombinants in a single step based on their ability to produce colour in the presence of a chromogenic substrate. In Recombinants, the inserted DNA fragment inactivates the lac z gene,as a result β-galactosidase enzyme is not produced ,the recombinant colonies appear colorless.In non recombinants ,the lacz gene is functional thus the β-galactosidase enzyme is expressed giving rise to blue colour colonies.(colorless substrate converted to blue color)

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