Q.

Insulin is dissolved in a suitable solvent and the osmotic pressure  (π) of solution of various concentrations (g/cm3) C is measured at 200C . The slope of  π against C is found to be 4.65×103 . The molecular weight of the insulin (g/mol) is: (R = 0.0821 lit atm  mole1K1)

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a

4.5×105

b

3×105

c

5.16×106

d

9×105

answer is D.

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Detailed Solution

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π=CRT. 

C = concentration in mole/lit

when expressed in gm/ cc

C=CMolar mass(M)×1000v(ml)

π  =CM×1000RT

Slope  =1000RTM

4.65×10-3=1000×0.0821×293M

M = 1000×0.0821×2934.65×10-3

 

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