Insulin is dissolved in suitable solvent and the osmotic pressure in atm of solutions at various concentration is measured at 20°C. The slope of the plot of p against C is found to be 4.65 × 10^-3. The molecular weight of insulin is 5.17 × 10^x. Then ‘x’ is

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One

five

two

six

answer is D.

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Detailed Solution

Applying Osmotic pressure

$π V = \frac{W B}{M B} \times R T$

$π = (\frac{W B}{V}) \times \frac{R T}{M B} \Rightarrow π = C \times \frac{R T}{M B} \times 100 . . . . (1)$

$While C = conc.in g / c c, 4 = x \times m . . . . (2)$

Combination equation 1 & 2

$Slope = \frac{R T}{M B} \times 1000$

$∴ M B = \frac{R T}{Slope} \times 1000 = \frac{0.0821 \times 293 \times 1000}{4.65 \times 10^{- 3}} = 5.17 \times 10^{6}$

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