Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
A. Insertion of recombinant DNA into the host cell.
B. Cutting of DNA at specific location by restriction enzyme.
C. Isolation of desired DNA fragment.
D. Amplification of gene of interest using PCR.
Choose the correct answer from the options given below:

Recombinant DNA technology involves several steps to be executed in a specific sequence. The sequence of steps are as follows: isolation of DNA, fragmentation of DNA by restriction endonucleases, isolation of a desired DNA fragment, amplification of gene of interest by PCR, ligation of the DNA fragment into a vector, transferring the recombinant DNA into the host, large scale culture of the host cells in a medium and extraction of the desired product. 

So, the sequence would be:

B. Cutting of DNA at specific location by restriction enzyme

C. Isolation of desired DNA fragment

D. Amplification of gene of interest using PCR

A. Insertion of recombinant DNA into the host cell