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Q.

Match the following columns and select the correct option.

 Column I Column II
(a)Bt cotton(i)Gene therapy
(b)Adenosine deaminase deficiency(ii)Cellular defense
(c)RNAi(iii)Detection of HIV infection
(d)PCR(iv)Bacillus thuringiensis
 abcd
1iiiiiiiv
2iiiiiivi
3iiiiiiiv
4iviiiiii

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a

2

b

1

c

3

d

4

answer is D.

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Detailed Solution

Bt cotton contain genes from Bacillus thuringiensis. Bt toxin gene has been cloned from the bacteria and been expressed in plants to provide resistance to insects without the need for insecticides; in effect created a bio-pesticide.

RNAi is a method of cellular defense in eukaryotes. This method involves silencing of a specific mRNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA (silencing).

Adenosine deaminase is a crucial enzyme for the functioning of immune system. Its deficiency leads to SCID (Severe combined immunodeficiency). It might be cured by gene therapy. 

PCR stands for Polymerase Chain Reaction.  PCR is now routinely used to detect HIV in suspected AIDS patients. In PCR, multiple copies of the gene (or DNA) of interest is synthesized in vitro using two sets of primers (small chemically synthesized oligonucleotides that are complementary to the regions of DNA) and the enzyme DNA polymerase.

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