Match  the  items  given  in  column-I  with  those  in column-II  and  choose  the  option  with  all  correct match.
 

	Column-I		Column-II
(A)	DNA ligase	(I)	Insertion of recombinant DNA into host cell
(B)	Agarose gel electrophoresis	(II)	Ligation of DNA fragment into a vector
(C)	Downstream processing	(III)	Separation of  DNA fragments
(D)	Transformation	(IV)	Separation of the product

	A	B	C	D
1	I	II	III	IV
2	IV	III	II	I
3	II	III	IV	I
4	II	IV	III	I