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Q.

Match the terms related to genetic cloning and selection (Column A) with their appropriate descriptions (Column B).

 Column  A Column  B
1)Single recognition sitesA)Site for foreign DNA insertion, impacting antibiotic resistance
2)BamH I siteB)Enzyme inactivated in recombinants to prevent color production
3)B-galactosidaseC)Confers survival on ampicillin medium, used for initial transformant selection
4)Ampicillin resistanceD)Preferred to minimize complexity in DNA cloning
 1234
1DABC
2DCBA
3ABCD
4BACD

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a

1

b

2

c

3

d

4

answer is A.

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Detailed Solution

 Column  A Column  B
1)Single recognition sitesD).  Preferred to minimize complexity in DNA cloning
2)BamH I siteA) Site for foreign DNA insertion, impacting antibiotic resistance
3)B-galactosidaseB)  Enzyme inactivated in recombinants to prevent color production 
4)Ampicillin resistanceC)Confers survival on ampicillin medium, used for initial transformant selection
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