Q.

Name the selectable marker that can differentiate recombinants from non-recombinants in a single step based on its ability to produce colour in the presenc eof chromogenic substrate?

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a

 lac z' gene

b

X gal gene

c

amp R gene

d

tet R gene

answer is B.

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Detailed Solution

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lac z' gene or β - gal  gene of pUC vectors undergo insertional inactivation if a foreign gene is inserted within this gene. As a result, it gives white colonies.

 If the lac z'  gene is intact, it gives blue colored colonies in the presence of chromogenic substrate -X -gal and inducer IPTG.

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Name the selectable marker that can differentiate recombinants from non-recombinants in a single step based on its ability to produce colour in the presenc eof chromogenic substrate?