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Q.

$(N a N O_{2} + H C l)$ converts an amino acid into hydroxyl acid with retention of configuration. Estimation of $N_{2}$ gas evolved in the reaction is the basis of van slyke estimation of amino acids. Which of the following cannot be analyzed by this method?

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a

Cysteine

b

Valine

c

Proline

d

Histidine

answer is B.

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Detailed Solution

Proline has $2^{\circ}$ amino group, which cannot be diazotized.

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(NaNO2+HCl) converts an amino acid into hydroxyl acid with retention of configuration. Estimation of N2 gas evolved in the reaction is the basis of van slyke estimation of amino acids. Which of the following cannot be analyzed by this method?