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Ninhydrin is a powerful oxidizing agent, and reacts with amino acids to form purple compound. This is used to identify spots in TLC in the separation of mixture of amino acids.

(Oxidation state of carbon is ‘+1’ that connected to oxygen atom in Y)
a = Total number of nitrogen atoms in ‘X’
b = total number of structural isomers of ‘Y’ in which oxidation state of carbon, connected to oxygen atom, is +1(excluding Y)
Report (a + b)

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NCERT (carbohydrates practical lab manual)
Page No : 9
(Replica)
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