Plasmid pBR322 has Pstl restriction enzyme site within gene amp^R that confers ampicillin resistance. If this enzyme is used for inserting a gene for β -galactoside production and the recombinant plasmid is inserted in an E.coli strain

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it will be able to produce a novel protien with dual ability.

it will lead to lysis of host cell.

it will not be able to confer ampicillin resistance to the host cell.

the transformed cells will have the ability to resist ampicilin as well as produce β -galactoside.

answer is B.

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Detailed Solution

Since the gene is inserted at Pst I of amp^R region of pBR322, the amp^R gene is inactivated which is called as insertional inactivation. Hence the genetically modified E.coli strain will not be able to confer ampicillin resistance.

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