Pyruvic acid before entering into Krebs cycle must be converted to Acetyl CoA. During the conversion of pyruvic acid to acetyl CoA, biological oxidation and decarboxylation must occur. The various cofactors required for the process are (Hint: AL= Lipoic acid, Acetyl CoA – Co enzyme A)

Pyruvic acid before entering into Krebs cycle must be converted to Acetyl CoA. The pyruvate dehydrogenase complex is a multimolecular aggregate of three enzymes, pyruvate decarboxylase (whose prosthetic group is coenzyme thiamine pyrophosphate TPP), dihydrolipoyl dehydrogenase (prosthetic group is lipoic acid) and dihydrolipoly dehydrogenase (prosthetic group is Flavin adenine dinucleotide FAD) , TPP, NAD, FAD, CoA , Lipoic acid and Mg²⁺ are the six cofactors.