Read the following statements and choose the option that correctly fills the blank (A) and (B).
(i) A gene gun is used to shoot high velocity microparticles of (A) , coated with DNA, into the plant protoplasts.
(ii) During heat shock method, bacterial cells are incubated with recombinant DNA on ice, followed by placing them briefly at a temperature of (B) , and then putting them back on ice.
A B
1. Silver 42ºC
2. Gold 72ºC
3. Tungsten 72ºC
4. Gold 42ºC

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answer is D.

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Detailed Solution

A gene gun is used to shoot high velocity micro-particles of gold or tungsten coated with DNA into the plant protoplasts. During heat shock method, bacterial cells are incubated with recombinant DNA on ice, followed by placing them briefly at a temperature of 42ºC and then putting them back on ice.

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