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Q.

Read the following statements

(i) Separation of source DNA fragments

(ii) Separation of RNA fragments

(iii) Separation of proteins

(iv) Separation of the vector from other DNA

(v) To check the progression of restriction enzyme digestion

Out of given events, how many can be achieved through agarose gel electrophoresis ?

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a

Two                          

b

Three

c

One                          

d

Five

answer is B.

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Detailed Solution


Agarose gel electrophoresis is a technique commonly used for the separation of macromolecules, such as DNA and RNA, based on their size. Let's evaluate each statement:

(i) Separation of source DNA fragments - Achievable through agarose gel electrophoresis.
(ii) Separation of RNA fragments - Agarose gel electrophoresis is more commonly used for DNA separation. RNA separation is typically done using polyacrylamide gel electrophoresis.
(iii) Separation of proteins - Agarose gel electrophoresis is not suitable for separating proteins. Polyacrylamide gel electrophoresis is commonly used for protein separation.
(iv) Separation of vector from other DNA - Achievable through agarose gel electrophoresis, especially if there is a size difference between the vector and other DNA fragments.
(v) To check the progression of restriction enzyme digestion - Achievable through agarose gel electrophoresis. This is often done to verify the success of DNA digestion by observing the appearance of specific bands.

Therefore, out of the given events, three can be achieved through agarose gel electrophoresis: (i) Separation of source DNA fragments, (iv) Separation of vector from other DNA, and (v) To check the progression of restriction enzyme digestion.

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