Q.

'Restriction' in restriction enzyme refers to

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a

cleaving of phosphodiester bond in DNA by the enzyme

b

Cutting of DNA at specific position only

c

prevention of the multiplication of bacteriophage in bacteria

d

restricting the recombinant DNA from multiplying in host cells

answer is C.

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Detailed Solution

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Restriction enzymes are the enzymes that cleave at specific DNA sequence. They are widely used in recombinant DNA technology. Restriction enzymes, are found in bacteria and archaea, are thought to have evolved to provide
a defense mechanism against invading viruses (bacteriophages). Inside a bacterial host, the restriction enzymes selectively cut foreign DNA in a process called restriction.

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