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Q.

Select the correct steps in preparing Bacterial staining
I) Put the drops of crystal violet on slide
II) Smear the bacteria on a slide and slightly heat the slide
III) Dry the slide and watch the slide under the microscope in 25 X or 40 X
IV) After 30 to 60 seconds gently wash the slide

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a

$II \to III \to I \to IV$

b

$III \to II \to I \to IV$

c

$II \to I \to IV \to III$

d

$II \to IV \to I \to III$

answer is C.

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Detailed Solution

Correct steps in preparing Bacterial staining smear the bacteria on a slide and slightly heat the slide, Put the drops of crystal violet on slide, After 30 to 60 seconds gently wash the slide, Dry the slide and watch the slide under the microscope in 25 X or 40 X

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