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Upto 900 restriction enzymes have been isolated from over 230 strains of bacteria.

In the year 1963, the two enzymes responsible for restricting the growth of bacteriophage in Escherichia coli were isolated.

Polymerase enzyme is one of the key tools for recombinant DNA technology.

EcoRI cut the DNA between bases G and A producing blunt ends, when the sequence GAATTC is present in the DNA.

answer is D.

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Detailed Solution

EcoRI identifies specific palindromic DNA sequence GAATTC and cuts are specific locations away from the center between G and A making staggered or sticky end cuts. It breaks phosphodiester bonds and hydrogen bonds.

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