Q.

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a

Selection of recombinants due to inactivation of antibiotic is a cumbersome procedure.

b

Insertional inactivation of β-galactosidase leads to colourless colonies.

c

Insertional inactivation of β-galactosidase leads to blue color colonies.

d

In insertional inactivation, the rDNA is inserted within the coding sequence of an enzyme β-galactosidase.

answer is C.

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Detailed Solution

Inactivation of the gene for synthesis of enzyme, is referred to as insertional inactivation. The presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert. Presence of insert results into insertional inactivation of the βgalactosidase gene and the colonies do not produce any colour, these are identified as recombinant colonies.

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Select the incorrect statement.