Select the incorrect statement.

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Selection of recombinants due to inactivation of antibiotic is a cumbersome procedure.

Insertional inactivation of β-galactosidase leads to colourless colonies.

Insertional inactivation of β-galactosidase leads to blue color colonies.

In insertional inactivation, the rDNA is inserted within the coding sequence of an enzyme β-galactosidase.

answer is C.

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Detailed Solution

Inactivation of the gene for synthesis of enzyme, is referred to as insertional inactivation. The presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert. Presence of insert results into insertional inactivation of the $β$ galactosidase gene and the colonies do not produce any colour, these are identified as recombinant colonies.

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