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Q.

Selectable marker gene in blue white screening method employing PUC 8 is

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a

penicillium resistant gene

b

tetracyclin resistant gene

c

kanamycin resistant gene

d

β - galactosidase encoding gene

answer is D.

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Detailed Solution

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in pUC series of vectors,β galactosidase is used as a selectable marker in blue white screening method. It helps the researcher to differentiate the transformed recombinants from non transformed colonies in a single step.

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