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Q.

Some foreign DNA fragment is attached to Cla I site of pBR 322. This recombinant vector is used to transform Escherichia coli. On two different media, one containing ampicillin and the other containing tetracycline, the transformed cells containing the recombinant vector will 

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a

Grow on ampicillin but not on tetracycline containing medium

b

Grow on tetracycline but not on ampicillin containing medium

c

Not grow on either tetracycline containing or ampicillin containing media

d

Grow on both, tetracycline and ampicillin containing media

answer is A.

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Detailed Solution

If foreign DNA fragment is attached to Cla I site of pBR 322. The recombinant vector is used to transform Escherichia coli. On two different media, one containing ampicillin and the other containing tetracycline, the transformed cells containing the recombinant vector will grow on both as Cla I is not present in selectable maker region.

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