Southern blotting is a technique in which

Southern blotting is a technique in which DNA fragments are transferred to synthetic membranes, such as nitrocellulose or nylon. This is followed by fragment detection by a radioactive probe hybridisation to identify the specific sequence in the DNA from the samples.

• Detection of the hybridized DNA fragments is called autoradiography.
• RNA transfer is called Northern blotting.
• Protein transfer is called Western blotting.