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Q.

Suppose the gene of interest is inserted at Bam H I site in pBR 322,the resulting plasmid will confer resistance to: 

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a

Ampicillin and tetracyclin 

b

Kanamycin and tetracyclin 

c

Ampicillin 

d

Tetracyclin

answer is D.

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Detailed Solution

If a gene is inserted at Bam H I site of pBR322, the tetR gene is inactivated and the host does not show resistance to antibiotic tetracyclin but still shows resistance to ampicillin. 

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Suppose the gene of interest is inserted at Bam H I site in pBR 322,the resulting plasmid will confer resistance to: