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Q.

The alien or foreign DNA coding for gene of interest can be made to replicate in host cells by linking to with the which region of plasmid?

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a

Ori

b

Multiple cloning site

c

Antibiotic resistance gene

d

β-galactosidase gene

answer is A.

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Detailed Solution

'Ori' or the origin of replication is the sequence where the replication starts in a plasmid. During cloning of a vector, it is important because any sequence linked to 'ori' in a plamid vector will get replicated. Ori controls the copy number of the linked DNA.

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The alien or foreign DNA coding for gene of interest can be made to replicate in host cells by linking to with the which region of plasmid?