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The anaphase promoting factor is activated when:

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Decondensation of chromosomes starts

Reassembly of nuclear membrane takes place

Spindle fibres attach to the chromosomes

Separation of sister chromatids begins

answer is C.

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Detailed Solution

After attachment of spindle fibres to chromosomes, anaphase promoting factor (APC) is activated. This APC is involved in:

(i) Separation of sister chromatids (division of centromere)

(ii) Decondensation of chromosome and reassembly of nuclear membrane

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