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Q.

The antibiotic resistance gene isolated by Cohen and Boyer was inserted in the plasmid found in which organism?

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a

E.coli

b

Salmonella typhimurium 

c

Saccharomyces cerevisiae

d

Bacillus subtilis

answer is B.

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Detailed Solution

The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of Salmonella typhimurium. Stanley Cohen and Herbert Boyer accomplished this in 1972 by isolating the antibiotic resistance gene by cutting out a piece of DNA from a plasmid which was responsible for conferring antibiotic resistance.  

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