The biosynthesis of phosphoenol pyruvic acid (PEP) occurs in mesophyll cells of C₄ plants in two methods at two different locations by

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phosphorylation in cytosol and hydration in chloroplast

carboxylation in chloroplast and hydration in cytosol

decarboxylation in chloroplast and oxidation in cytosol

phosphorylation in chloroplast and dehydration in cytosol

answer is A.

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Detailed Solution

In mesophyll cells, phosphoenolpyruvate (PEP) is synthesized from pyruvate and ATP by the enzyme pyruvate phosphate dikinase (PPDK). PPDK catalyzes the phosphorylation of pyruvate to form phosphoenolpyruvate (PEP) in the chloroplasts of mesophyll cells.

During glycolysis, PEP is formed from 2-phosphoglycerate by a dehydration reaction catalyzed by the enzyme enolase in the cytosol.

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