Q.

The enzyme ligase is not involved in one of the following activity

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a

To join the source DNA and vector

b

To join the antibiotic  resistance gene with the plasmid

c

Fragmentation of desired DNA

d

Joining of Okajaki fragments

answer is D.

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Detailed Solution

Ligas helps on formation of phosphodiester bonds, but it does not brake the bonds

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The enzyme ligase is not involved in one of the following activity