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Q.

The following illustrates the linking of DNA fragments.
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(a) Write the name of A and B.
(b) Complete the palindrome, which is recognised by EcoRI and write the name of the enzyme that can link the two DNA fragments.

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Detailed Solution

(a) A–Vector DNA/Plasmid DNA, B–Foreign DNA
(b) 5’ –GAATTC–3’; 3’–CTTAAG–5’ is the palindromic sequence recognised by EcoRI and DNA ligase is used to link the two DNA fragments.

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The following illustrates the linking of DNA fragments.(a) Write the name of A and B.(b) Complete the palindrome, which is recognised by EcoRI and write the name of the enzyme that can link the two DNA fragments.