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The given figure is the diagrammatic representation of the vector pBR322. Which one of the given options correctly identifies its certain component(s)?

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ori–original restriction enzymes

ampR, tetR–antibiotic resistant genes

rop–reduced osmotic pressure

Hind III, Eco RI–selectable markers

answer is D.

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Detailed Solution

The genes encoding resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin is often used as a selectable marker. Therefore, amp^Rand tet^R represents antibiotic resistant genes which acts as selectable markers.

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