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Q.

The last step in the protocol for DNA fingerprinting is

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a

autoradiography

b

blotting 

c

electrophoresis

d

hybridisation

answer is C.

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Detailed Solution

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The correct sequence of steps in DNA fingerprinting is:

  1. Isolation of DNA
  2. Digestion of DNA by restriction endonucleases
  3. Separation of DNA fragments by electrophoresis
  4. Transferring (blotting) of separated DNA fragments to synthetic membranes
  5. Hybridisation using labelled VNTR probe
  6. Detection of hybridised DNA fragments by autoradiography
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