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Q.

The most frequently used membrane for Southern blotting is made up of

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a

polyethylene glycol

b

nitrocellulose 

c

polyvinyl chloride

d

ethidium bromide

answer is D.

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Detailed Solution

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Nitrocellulose is a type of filter paper that is commonly used in blotting techniques, such as Southern blotting and Western blotting. Blotting techniques are used to transfer molecules, such as DNA or proteins, from a gel or other solid matrix to a solid support, such as a membrane or filter paper. Nitrocellulose is highly absorbent and has a pore size that allows for the efficient transfer of molecules. It is also stable and can be stored for long periods of time without degrading.
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