The plasmid pBR322 contains a PstI restriction site within the ampicillin resistance gene (ampR). When Pstl is employed to insert a desired gene responsible for beta-galactosidase production, what would be the outcome once the recombinant plasmid is introduced into an E.coli strain

The E.coli will lose the ability to resist ampicillin due to disruption of the ampR gene..

The E.coli will not gain the novel function of producing beta-galactosidase 

The insertion of the gene will result in the lysis of the E.coli cells

The E.coli will be transformed, exhibiting resistance to ampicillin and producing beta-galactosidase simultaneously.