The primers used in the PCR technique should be

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complementary to the 5'-end sequence of the DNA segment to be amplified.

formed of polypeptide

polynucleotide chain tagged with radioisotope.

complementary to the 3'-end sequence of the DNA segment to be amplified.

answer is C.

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Detailed Solution

In PCR, multiple copies of the gene (or DNA) of interest is synthesised in vitro using two sets of primers, they are small chemically synthesised oligonucleotides that are complementary to the 3' - end regions of DNA and the enzyme DNA polymerase.

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