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The specific palindromic sequence which is recognized by EcoRI is :

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5’-CTTAAG-3’
3’-GAATTC-5’

5’-GGATCC-3’
3’-CCTAGG-5’

5’-GAATTC-3’
3’-CTTAAG-5’

5’-GGAACC-3’
3’-CCTTGG-5’

answer is D.

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Detailed Solution

EcoRI, a restriction enzyme, recognises the palindromic sequence
5’ . . . . G A A T T C. . . . 3’
3’ . . . . C T T A A G. . . . 5’

(2) 5’ . . G G A A C C. . 3’; 3’. . C C T T G G . . 5’ is not a palindromic sequence.

(3) 5’..C T T A A G. . 3’; 3’.. G A A T T C..5’ is recognised by AflII enzyme.

(4) 5’. . G G A T C C..3’; 3’..C C T A G G. .5’ is recognised by AsiI sequence.

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