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Q.

The substituents R1 and R2 for nine peptides are listed in the table given below. How many of these peptides are positively charged at pH = 7.0?

Question Image
PeptideR1R2
IHH
IIHCH3
IIICH2COOHH
IVCH2CONH2(CH2)4NH2
VCH2CONH2CH2CONH2
VI(CH2)4NH2(CH2)4NH2
VIICH2COOHCH2CONH2
VIIICH2OH(CH2)4NH2
IX(CH2)4NH2CH3

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answer is 4.

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Detailed Solution

Peptides with isoelectric point (pI) more than 7 would exist as cation in neutral solution (pH = 7) which means the given polypeptide is of basic nature, so it must contain two or more amino groups. hence IV, VI, VIII and IX are correct options

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