The term “elution” refers to

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cutting out of the separated band of DNA from Agarose gel and extraction from gel piece

joining of DNA fragment to the cloning vector

separation of purified DNA by spooling

staining of DNA fragments with ethidium bromide

answer is D.

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Detailed Solution

In molecular biology, the term "elution" refers to the process of removing a substance from a solid support, such as an agarose gel, using a solvent. In the context of DNA analysis, elution typically refers to the process of extracting a purified DNA fragment from an agarose gel after it has been separated by electrophoresis.

After electrophoresis, the DNA fragments are visualized using a staining agent such as ethidium bromide, and the fragment of interest is cut out of the gel using a razor blade or other tool. The fragment is then extracted from the gel piece using a solvent such as TE buffer or water through a process known as elution.

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