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Q.

The value of numerical aperture of the objective lens of a microscope is 1.25. If light of wavelength 5000Ao  is used, the minimum separation between two points, to be seen as distinct, will be

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a

0.38μm

b

0.12μm

c

0.24μm

d

0.48μm

answer is A.

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Detailed Solution

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Numerical aperture of the microscope is given as
NA=0.61λd
Where d=  minimum separation between two points to be seen as distinct
⇒d=0.61λNA=(0.61)×(5000×10−10)m1.25
⇒d=2.4×10−7m

⇒d=0.24μm

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