This may not necessarily indicate inflammation.

 

Concept: Opsonization is an immunological procedure in which opsonins are used to mark foreign pathogens for phagocyte eradication. 

The negatively charged cell walls of the pathogen and phagocyte reject one another in the absence of an opsonin, such as an antibody. 

Opsonization is a crucial step in host defense that prepares particles or complexes for easy ingestion by phagocytic cells.

Hence, option 4 is correct answer