‘TRIS’ buffer which is a mixture of a primary amine, (CH2OH)3CNH2 and its salt, (CH2OH)3CNH3+Cl− has found extensive use in clinical chemistry. For the preparation of 500 mL 0.5M ‘TRIS’ buffer of pH = 7.4, ‘x’ grams of solid amine and ‘v’ mL of 1.0 M HCl is required.[ Given that, pKa of (CH2OH)3CNH3+Cl−=8.0, log2=0.30, pKw=14, Molecular weight of (CH2OH)3CNH2=121g/mol ]

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‘TRIS’ buffer which is a mixture of a primary amine, ${(C H_{2} O H)}_{3} C N H_{2}$ and its salt, ${(C H_{2} O H)}_{3} C N H_{3}^{+} C l^{-}$ has found extensive use in clinical chemistry. For the preparation of 500 mL 0.5M ‘TRIS’ buffer of pH = 7.4, ‘x’ grams of solid amine and ‘v’ mL of 1.0 M HCl is required.
[ Given that, $p K_{a}$ of ${(C H_{2} O H)}_{3} C N H_{3}^{+} C l^{-} = 8.0, \log 2 = 0.30, p K_{w} = 14,$ Molecular weight of ${(C H_{2} O H)}_{3} C N H_{2} = 121 g / m o l$ ]