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Two isomers A and B can be separated from their mixture if the energy barrier is

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a

0.6 Kcal/mole

b

9 Kcal/mole

c

16 Kcal/mole

d

12 Kcal/mole

answer is C.

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Detailed Solution

The energy barrier is caused by atoms rotating along carbon-carbon bonds due to their geometrical orientation.
For the separation of two isomers $A$ and $B$ from their mixture, the energy barrier must be $16 Kcal / mole$ .
The eclipsed conformation is the least stable with the maximum energy whereas the staggered conformation is relatively more stable with less energy.

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