Q.

Upon exposure to UV radiation, DNA stained with ethidium bromide will show

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a

bright blue colour

b

bright red colour

c

bright yellow colour

d

bright orange colour

answer is A.

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Detailed Solution

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The separated DNA fragments in agarose gel electrophoresis are visualised using a fluorescent stain, ethidium bromide. The stain, being an intercalating agent, sits between the two strands of a DNA molecule. Upon exposure to UV light, the stain fluoresces as bright, orange-coloured bands.


(2),(3) and (4) DNA stained with ethidium bromide will not show bright red colour, bright blue colour or bright yellow colour when exposed to UV radiation.

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Upon exposure to UV radiation, DNA stained with ethidium bromide will show