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Q.

What happens to recombinant bacterial colonies that contain an insert disrupting the β-galactosidase enzyme coding sequence when they are exposed to a chromogenic substance in the culture medium?

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a

They produce a red colour

b

They produce both lactose and galactose.

c

They produce a blue colour

d

They do not produce any colour

answer is A.

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Detailed Solution

Since the recombinant colonies cannot express the galactisidase enzyme,the chromogenic substrate cannot be cleaved as a result the recombinant colonies appear white.

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