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What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium ?
I. Transformed bacteria with a recombinant DNA molecule
II. Cut the plasmid DNA using restriction enzymes
III Extract plasmid DNA from bacterial cells
lV. Use ligase to seal plasmid DNA and foreign DNA

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An Intiative by Sri Chaitanya

I → II → III → IV

II → I → III → IV

III → II → IV → I

III → IV → I → II

answer is A.

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Detailed Solution

The correct sequence is isolation of plasmid, opening the plasmid, inserting the gene into plasmid to form rDNA by using the ligase and incorporating the same into the bacterial host by transformation.

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