Q.

What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium ? 

I. Transformed bacteria with a recombinant DNA molecule 

II. Cut the plasmid DNA using restriction enzymes 

III Extract plasmid DNA from bacterial cells 

lV. Use ligase to seal plasmid DNA and foreign DNA

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a

III → II → IV → I

b

III → IV → I → II 

c

I → II → III → IV

d

II → I → III → IV

answer is A.

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Detailed Solution

The correct sequence is isolation of plasmid, opening the plasmid, inserting the gene into plasmid to form rDNA by using the ligase and incorporating the same into the bacterial host by transformation.

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What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium ? I. Transformed bacteria with a recombinant DNA molecule II. Cut the plasmid DNA using restriction enzymes III Extract plasmid DNA from bacterial cells lV. Use ligase to seal plasmid DNA and foreign DNA